check quantity

LANCE® Eu-W1024 phospho-Akt substrate antibody, 10 µg

Europium-labeled anti-phospho-Akt substrate antibody, for use in LANCE® TR-FRET kinase assays.

Please enter valid quantity

Please login to add favorites


Product Number List Price Your Price Quantity
AD0184 4201.00 USD

You successfully added item(s) to your cart


Phospho-Akt substrate antibody is an affinity-purified rabbit polyclonal antibody (supplied by Cell Signaling Technology). It is highly specific for phosphorylated threonine preceded by arginine at positions -5 and -3. Some cross-reactivity is observed for phospho-serine with arginine at positions -5 and -3 or -3 and -2.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Antibody Conjugates Anti-P-Akt
Automation Compatible Yes
Detection Method TR-FRET, Time-Resolved Fluorescence (TRF)
Experimental Type In vitro
Fluorophore Eu-W1024
Molecular Modification Phosphorylation
One Unit Contains 10 µg
Product Brand Name LANCE
Shipping Condition Blue Ice
Unit Size 10 µg

Resource Library

Resource Type File Name File Format
Application Note How to Optimize a Tyrosine Kinase Assay Using Time Resolved Fluorescence-Based LANCE™ Detection PDF 907 KB
Application Note Measurement of p38/MAPK Activity Using LANCE PDF 632 KB