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LANCE® Eu-W1024 phospho-Akt substrate antibody, 10 µg

Europium-labeled anti-phospho-Akt substrate antibody, for use in LANCE® TR-FRET kinase assays.

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AD0184 4201.00 USD

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Overview

Phospho-Akt substrate antibody is an affinity-purified rabbit polyclonal antibody (supplied by Cell Signaling Technology). It is highly specific for phosphorylated threonine preceded by arginine at positions -5 and -3. Some cross-reactivity is observed for phospho-serine with arginine at positions -5 and -3 or -3 and -2.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Specifications

Antibody Conjugates Anti-P-Akt
Automation Compatible Yes
Detection Method TR-FRET, Time-Resolved Fluorescence (TRF)
Experimental Type In vitro
Fluorophore Eu-W1024
Molecular Modification Phosphorylation
Product Brand Name LANCE
Shipping Condition Blue Ice
Unit Size 10 µg

Resources, Events & More

Resource Type File Name File Format
Application Note How to Optimize a Tyrosine Kinase Assay Using Time Resolved Fluorescence-Based LANCE™ Detection PDF 907 KB
Application Note Measurement of p38/MAPK Activity Using LANCE PDF 632 KB