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LANCE Eu-W1024 Anti-Human IgG, 50µg

Europium-labeled anti-human IgG, for capturing antibodies in LANCE® TR-FRET assays.

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Product Number Unit Size List Price Your Price Quantity
AD0212 10 µg 199.00 USD
AD0074 50 µg 983.00 USD
AD0075 1 mg 8600.00 USD

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Eu-W1024 labeled anti-human IgG antibodies for LANCE® assays where analyte concentration is < 1-10 nmol/L and assay pH > 7. This affinity purified mouse monoclonal IgG2b antibody is specific for the Fc-part of human IgG. The antibodies recognize all IgG subclasses and cross-react with rabbit immunoglobulins. It is supplied ready to use in 50 mmol/L Tris-HCl buffered saline solution.

Generic LANCE® reagents are intended for setting up homogeneous time-resolved fluorescence resonance energy transfer (TR-FRET) based assays using Eu-Chelate label as a donor and APC-labeled reagent as an acceptor. Generic reagents facilitate setting up LANCE® assays when there is a limited amount of specific reagents available or the assay reagents are relatively unstable. To enable optimization of both the signal or the noise ratio and signal stability, some of the generic reagents are labeled with two different chelates, W1024 or W8044.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Antibody Conjugates Anti-human IgG
Automation Compatible Yes
Detection Method TR-FRET, Time-Resolved Fluorescence (TRF)
Experimental Type In vitro
Fluorophore Eu-W1024
One Unit Contains 50 µg
Product Brand Name LANCE
Shipping Condition Blue Ice
Unit Size 50 µg

Resource Library & Events

Resource Type File Name File Format
Manual LANCE generic reagents PDF 101 KB