Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in LANCE® TR-FRET assays.
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|Product Number||Unit Size||List Price||Your Price||Quantity|
|AD0205||10 µg||229.00 USD|
|AD0110||50 µg||1085.00 USD|
|AD0111||1 mg||10800.00 USD|
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Eu-W1024 labeled anti-6xHis antibodies for LANCE® assays.The anti-6xHis antibody is a purified mouse IgG1 monoclonal antibody. It is raised against a polypeptide containing a 6X histidine tag. The antibody has been shown to detect polyhistidine tags localized at the amino- or carboxyl-terminus.
LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).
|Antibody Conjugates||Anti-6X His|
|Detection Method||TR-FRET, Time-Resolved Fluorescence (TRF)|
|Experimental Type||In vitro|
|Product Brand Name||LANCE|
|Shipping Condition||Dry Ice|
|Unit Size||50 µg|
|Resource Type||File Name||File Format|
|Guide||LANCE Protein: Protein Interaction Quick Start Guide||PDF 1 MB|
|Manual||LANCE generic reagents||PDF 101 KB|