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LANCE Eu-W1024 Anti-6xHis, 50 µg

Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in LANCE® TR-FRET assays.

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Product Number Unit Size List Price Your Price Quantity
AD0205 10 µg 222.00 USD
AD0110 50 µg 1053.00 USD
AD0111 1 mg 10500.00 USD

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Overview

Eu-W1024 labeled anti-6xHis antibodies for LANCE® assays.The anti-6xHis antibody is a purified mouse IgG1 monoclonal antibody. It is raised against a polypeptide containing a 6X histidine tag. The antibody has been shown to detect polyhistidine tags localized at the amino- or carboxyl-terminus.

LANCE® (Lanthanide chelate excite) and LANCE® Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One molecule of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore [ULight™ dye, allophycocyanin (APC), etc.]. Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm (for ULight dye and APC).

Specifications

Antibody Conjugates Anti-6X His
Automation Compatible Yes
Detection Method TR-FRET, Time-Resolved Fluorescence (TRF)
Experimental Type In vitro
Fluorophore Eu-W1024
Product Brand Name LANCE
Shipping Condition Dry Ice
Unit Size 50 µg

Resources, Events & More

Resource Type File Name File Format
Manual LANCE generic reagents PDF 101 KB