LANCE® TR-FRET cAMP assay kit, for Gs- and Gi-coupled GPCR functional assays.
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LANCE cAMP Assay. Red, robust and ready for uHTS! Measure cAMP produced by whole cells or membrane receptors stimulated with GPCR agonists and antagonists with more sensitivity and precision than ever before and outstanding productivity.
PerkinElmer's innovative LANCE® cAMP assay combines homogeneous TR-FRET technology with red-shifted Alexa® Fluor dye chemistry for maximum excitation/emission discrimination. The result is a FRET assay without the compound interference associated with blue dyes. You can read longer without accumulating background signal to maximize S/N and Z'.
LANCE cAMP Assay Principle. Light pulse at 340 nm excites the Europium-chelate of the Eu-SA/b-cAMP tracer. The energy emitted from the chelate is transferred to the Alexa-labeled antibodies bound to the tracer, generating a TR-FRET signal at 665 nm. Residual energy from the chelate will produce light at 615 nm. cAMP of a sample competes with the tracer for antibody binding sites and causes signal reduction. This superior performance and ultra high throughput screening convenience leads to dramatic cost savings.
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Experimental Type||In vitro|
|Product Brand Name||LANCE|
|Quantity in a Package Amount||1000.0 Units|
|Second Messenger Release||cAMP|
|Shipping Condition||Blue Ice|
|Unit Size||1,000 Assay Points|
G-protein coupled receptors(GPCRs) continue to represent the most important class of pharmaceutical targets. Their activity is commonly assessed by measuring the intracellular second messengers released upon their stimulationby appropriate agonists. Cyclic AMP (cAMP) is one of thesecond messengers frequently measured to find and characterize both agonist and antagonist drug leads.
This data sheet describes LANCETM Normalization using Wallac brand instruments. LANCE normalization can be performed either by using WIZARD software, which will guides us through the process or the Normalization samples which are run among other samples i
LANCE Controls are intended for in vitro use for testing instrument performance in LANCE measurements.
This LANCE™ cAMP 384 kit is intended for the quantitative determination of adenosine 3',5'-cyclic monophosphate (cAMP) in cell culture and cell membrane samples.