LANCE® TR-FRET cAMP assay kit, for Gs- and Gi-coupled GPCR functional assays.
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|Product Number||Unit Size||List Price||Your Price||Quantity|
|AD0262||500 assay points||158.00 USD|
|AD0262E||1,000 assay points||509.00 USD|
|AD0263||10,000 assay points||1580.00 USD|
|AD0264||50,000 assay points||7000.00 USD|
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LANCE cAMP Assay. Red, robust and ready for uHTS! Measure cAMP produced by whole cells or membrane receptors stimulated with GPCR agonists and antagonists with more sensitivity and precision than ever before and outstanding productivity.
PerkinElmer's innovative LANCE® cAMP assay combines homogeneous TR-FRET technology with red-shifted Alexa® Fluor dye chemistry for maximum excitation/emission discrimination. The result is a FRET assay without the compound interference associated with blue dyes. You can read longer without accumulating background signal to maximize S/N and Z'.
LANCE cAMP Assay Principle. Light pulse at 340 nm excites the Europium-chelate of the Eu-SA/b-cAMP tracer. The energy emitted from the chelate is transferred to the Alexa-labeled antibodies bound to the tracer, generating a TR-FRET signal at 665 nm. Residual energy from the chelate will produce light at 615 nm. cAMP of a sample competes with the tracer for antibody binding sites and causes signal reduction. This superior performance and ultra high throughput screening convenience leads to dramatic cost savings.
|Detection Method||TR-FRET, Time-Resolved Fluorescence (TRF)|
|Experimental Type||In vitro|
|Product Brand Name||LANCE|
|Second Messenger Release||cAMP|
|Shipping Condition||Blue Ice|
|Unit Size||50,000 assay points|
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|Resource Type||File Name||File Format|
|Application Note||A Comparison of PerkinElmer's LANCE cAMP Assay Performance to that of State of the Art Competitive Technologies||PDF 655 KB|
|Application Note||Quench Correction for LANCE Time-Resolved Fluorescence Resonance Energy Transfer||PDF 847 KB|
|Manual||LANCE™ cAMP 384 Kit||PDF 244 KB|
|Data Sheet||LANCE Controls - For Service Purposes Only - AD0163||PDF 231 KB|
|Poster||Comparison of Kinase Assay Technologies for High Throughput Screening||PDF 406 KB|
|Poster||Kinetic Analysis of Receptor binding Measured by Scintillation proximity Assay (SPA)||PDF 221 KB|
|Poster||LANCE™ cAMP + Evolution™ P3 + ViewLux™ uHTS||PDF 174 KB|
|Poster||LANCE™ cAMP: Increased Sensitivity, Stability and Overall Assay Performance with Gas and Gai Coupled Receptors in a Miniaturized 1536-Well Format||PDF 304 KB|