LANCE® TR-FRET cAMP assay kit, for Gs- and Gi-coupled GPCR functional assays.
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LANCE cAMP Assay. Red, robust and ready for uHTS! Measure cAMP produced by whole cells or membrane receptors stimulated with GPCR agonists and antagonists with more sensitivity and precision than ever before and outstanding productivity.
PerkinElmer's innovative LANCE® cAMP assay combines homogeneous TR-FRET technology with red-shifted Alexa® Fluor dye chemistry for maximum excitation/emission discrimination. The result is a FRET assay without the compound interference associated with blue dyes. You can read longer without accumulating background signal to maximize S/N and Z'.
LANCE cAMP Assay Principle. Light pulse at 340 nm excites the Europium-chelate of the Eu-SA/b-cAMP tracer. The energy emitted from the chelate is transferred to the Alexa-labeled antibodies bound to the tracer, generating a TR-FRET signal at 665 nm. Residual energy from the chelate will produce light at 615 nm. cAMP of a sample competes with the tracer for antibody binding sites and causes signal reduction. This superior performance and ultra high throughput screening convenience leads to dramatic cost savings.
|Detection Method||Time-Resolved Fluorescence (TRF), TR-FRET|
|Experimental Type||In vitro|
|Product Brand Name||LANCE|
|Quantity in a Package Amount||10000.0 Units|
|Second Messenger Release||cAMP|
|Shipping Condition||Blue Ice|
|Unit Size||10,000 Assay Points|