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IgG (rabbit) LANCE Ultra TR-FRET Detection Kit, 500 Assay Points

The LANCE® Ultra Rabbit IgG Detection Kit is designed for detection and quantitation of rabbit IgG in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

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Part Number Unit Size List Price Your Price Quantity
TRF1104C 500 Assay Points 773.00 USD
TRF1104M 10,000 Assay Points 10300.00 USD
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Overview

The LANCE® Ultra Rabbit IgG Detection Kit is designed for detection and quantitation of rabbit IgG in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

 

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

 

The glycoprotein immunoglobulin G (IgG), a major effector molecule of the humoral immune response in rabbit, accounts for the majority of the total immunoglobulins in plasma whereas IgM, IgA, and IgE, each of which has characteristic properties and functions, constitute the remainder The basic IgG molecule has a four-chain structure, comprising two identical heavy (H) chains and two identical light (L) chains, linked together by interchain disulfide bonds.. The present kit recognizes total IgGs Fc fragment.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target IgG
Assay Target Class Antibody
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Rabbit
Therapeutic Area Biologics/Bioprocess
Unit Size 500 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Application Note High Throughput Quantitation of Cytokine Biomarkers using LANCE Ultra TR-FRET Assays PDF  1 MB
Application Note Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET PDF  3 MB
Manual LANCE Ultra Rabbit IgG Detection Kit TRF1104 PDF  494 KB