Our stable recombinant AequoScreen cell lines express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light.
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AequoScreen® Double Transfected Cell Line: Human recombinant lysophospholipid, S1P2 (Edg5) subtype receptor in aequorin CHO-K1 host cell. We provide two vials of cryopreserved cells (approximately 2.5x106 cells/vial), detailed product information including cell line properties, culture conditions and the pharmacological properties of the recombinant channel in a functional assay. All cell lines are tested for the absence of mycoplasma. Terms and conditions apply. Some of our receptors may be restricted for sale in specified countries. Please inquire at your local sales office for more information.
The AequoScreen technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.
|Assay Validated||Calcium Luminescence|
|Second Messenger Release||Calcium flux|
|Shipping Condition||Dry Ice|
|Unit Size||5 million cells|