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Secretin AequoScreen Cell Line

Our stable recombinant AequoScreen cell lines express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light.

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Product Number
ES-712-A

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Overview

AequoScreen® Double Transfected Cell Line: Human recombinant Secretin receptor in aequorin CHO-K1 host cell. We provide two vials of cryopreserved cells (approximately 2.5 x 106 cells/vial), detailed product information including cell line properties, culture conditions and the pharmacological properties of the recombinant receptor in a functional assay. All cell lines are tested for the absence of mycoplasma. Terms and conditions apply. Some of our receptors may be restricted for sale in specified countries. Please inquire at your local sales office for more information.

Features:

  • Fully automatable and miniaturizable to 1536
  • Enables reduction in reagents and consumable costs
  • High signal to background ratio enabling detection of agonists, antagonists and allosteric modulators
  • Robust assay (Z', CV values, solvent resistance)
  • Reduces the number of false positives from autofluorescent compounds in screening libraries
  • Option to work with cells in suspension to increase throughput
  • Amenable for use with calcium coupled ion channels and tyrosine kinase receptors

The AequoScreen technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.

Disclaimer: This product contains living cells. Some cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information.

Specifications

Assay Target Class GPCR
Assay Target Type Cell line
Assay Validated Calcium Luminescence
G-Alpha Natural Receptor Gs
Host Cell CHO-K1
Product Brand Name AequoScreen
Receptor Type Glucagon
Second Messenger Release Calcium flux
Shipping Condition Dry Ice
Therapeutic Area Metabolic
Unit Size 5 million cells