Our frozen, irradiated AequoZen cells express a variety of GPCRs which all couple to a calcium response. Upon GPCR stimulation and subsequent calcium binding to the aequorin oxidation of coelenterazine leads to emission of light. No cell culture is required prior to testing, for these frozen cells.
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Frozen Cells validated for calcium testing: Free Fatty Acid, FFA1 (GPR40) subtype, human recombinant, in CHO-K1 host cell. We provide one vial of frozen cells (10 million cells/vial). Some of our Frozen cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information. You are authorized to utilize these frozen cell preparations one time only. Any attempt to transfer, re-use, or propagate these cells is expressly unauthorized and a violation of the product terms and conditions of sale.
The AequoScreen technology is a generic GPCR technology which can be used with Gs, Gi and Gq coupled GPCRs and calcium coupled ion channels. Following receptor stimulation, increases in intracellular calcium enable measurement of the resulting flash luminescence signal.
The frozen cells approach to functional testing consists in the dissociation of cell culture from testing. That means that we will culture the cells and freeze them with an optimized protocol to stop all replication. These cells are then stored in liquid nitrogen or simply in a -80°C freezer, thawed and used directly in a functional, cellular GPCR test with previously validated performance.
Disclaimer: This product contains living cells. Some AequoZen™ FroZen Cells may be restricted for sale in specified countries. Please inquire at your local sales office for more information.
|Assay Target Class||GPCR|
|Assay Target Type||Cell line|
|Assay Validated||Calcium Luminescence|
|G-Alpha Natural Receptor||Gq/G11|
|Product Brand Name||AequoZen|
|Receptor Type||Free Fatty Acid|
|Second Messenger Release||Calcium flux|
|Shipping Condition||Dry Ice|
|Therapeutic Area||Metabolic, Cancer|
|Unit Size||10 million cells|
|Resource Type||File Name||File Format|
|Brochure||EnSpire Multimode Plate Reader with Label-free Technology Brochure||PDF 4 MB|
|Application Note||AequoScreen aequorin-encoding Plasmids for performing aequorin luminescent assay starting from non-aequorin cell lines||PDF 607 KB|
|Application Note||Aequorin bioluminescence assays on MicroBeta2 LumiJET for studying Ca2+-coupled GPCRs and ion channels||PDF 152 KB|
|Application Note||Pathway independent cell-based assays on the EnSpire Multimode Plate Reader with Epic® label-free technology||PDF 1 MB|
|Flyer||Aequorin FLIPR||PDF 1 MB|