DELFIA® TRF (time-resolved fluorescence) cytotoxicity kit for measuring chemical cytotoxicity, cell-mediated cytotoxicity, and ADCC.
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DELFIA® cytotoxicity assays offer a non-radioactive method to be used in cell mediated cytotoxicity studies. The method is based on loading cells with an acetoxymethyl ester of a fluorescence enhancing ligand. After the ligand has penetrated the cell membrane the ester bonds are hydrolyzed within the cell to form a hydrophilic ligand, which no longer passes through the membrane. After cytolysis the released ligand is introduced to a europium solution to form a fluorescent chelate. The measured signal correlates directly with the amount of lysed cells.
Reagents for the assay are also available separately: 4005-0010 Lysis buffer (30 mL), C135-100 DELFIA Eu-Solution (200mL), C136-100 DELFIA BATDA labeling reagent (50µL).
|Detection Method||DELFIA TRF, Time-Resolved Fluorescence (TRF)|
|Experimental Type||In vitro|
|Product Brand Name||DELFIA|
|Shipping Condition||Blue Ice|
|Unit Size||1 Kit|
|Resource Type||File Name||File Format|
|Application Note||DELFIA Cytotoxicity||PDF 497 KB|
|Application Note||DELFIA VICTOR Immunoassays- How to Optimize Rapid and Simple Immunoassays||PDF 406 KB|
|Manual||Microsoft Word - AD0116_en.doc||PDF 55 KB|