check quantity

DELFIA® EuTDA Cytotoxicity Reagents

DELFIA® TRF (time-resolved fluorescence) cytotoxicity kit for measuring chemical cytotoxicity, cell-mediated cytotoxicity, and ADCC.

Please enter valid quantity

Please login to add favorites

NULL OR EMPTY CART

Product Number List Price Your Price Quantity
AD0116 383.00 USD

You successfully added item(s) to your cart

Overview

DELFIA® cytotoxicity assays offer a non-radioactive method to be used in cell mediated cytotoxicity studies. The method is based on loading cells with an acetoxymethyl ester of a fluorescence enhancing ligand. After the ligand has penetrated the cell membrane the ester bonds are hydrolyzed within the cell to form a hydrophilic ligand, which no longer passes through the membrane. After cytolysis the released ligand is introduced to a europium solution to form a fluorescent chelate. The measured signal correlates directly with the amount of lysed cells.

Reagents for the assay are also available separately: 4005-0010 Lysis buffer (30 mL), C135-100 DELFIA Eu-Solution (200mL), C136-100 DELFIA BATDA labeling reagent (50µL).

Specifications

Automation Compatible Yes
Detection Method DELFIA TRF, Time-Resolved Fluorescence (TRF)
Experimental Type In vitro
Product Brand Name DELFIA
Shipping Condition Blue Ice
Unit Size 1 Kit

Resources, Events & More

Resource Type File Name File Format
Application Note DELFIA Cytotoxicity PDF 497 KB
Application Note DELFIA VICTOR Immunoassays- How to Optimize Rapid and Simple Immunoassays PDF 406 KB
Manual Microsoft Word - AD0116_en.doc PDF 55 KB