PerkinElmer
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DELFIA® EuTDA Cytotoxicity Reagents

DELFIA® TRF (time-resolved fluorescence) cytotoxicity kit for measuring chemical cytotoxicity, cell-mediated cytotoxicity, and ADCC.

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AD0116 394.00 USD

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Overview

DELFIA® cytotoxicity assays offer a non-radioactive method to be used in cell mediated cytotoxicity studies. The method is based on loading cells with an acetoxymethyl ester of a fluorescence enhancing ligand. After the ligand has penetrated the cell membrane the ester bonds are hydrolyzed within the cell to form a hydrophilic ligand, which no longer passes through the membrane. After cytolysis the released ligand is introduced to a europium solution to form a fluorescent chelate. The measured signal correlates directly with the amount of lysed cells.

Reagents for the assay are also available separately: 4005-0010 Lysis buffer (30 mL), C135-100 DELFIA Eu-Solution (200mL), C136-100 DELFIA BATDA labeling reagent (50µL).

Specifications

Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), DELFIA TRF
Experimental Type In vitro
Product Brand Name DELFIA
Quantity in a Package Amount 960.0 Units
Shipping Condition Blue Ice
Unit Size 1 Kit

Resources, Events & More

Resource Type File Name File Format
Brochure Drug Discovery Screening Solutions Brochure PDF 880 KB
Application Note A Simplified, Gentle Cell-Labelling Method for Non-Radioactive Cytotoxicity Assays PDF 497 KB
Application Note DELFIA VICTOR Immunoassays- How to Optimize Rapid and Simple Immunoassays PDF 406 KB
Manual Microsoft Word - AD0116_en.doc PDF 55 KB