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DELFIA® Eu-Solution, 200mL (for Cytotoxicity Detection Only)

DELFIA® Europium solution for use in time-resolved fluorescence (TRF) cytotoxicity assays.

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Product Number List Price Your Price Quantity
C135-100 161.00 USD

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Overview

The DELFIA europium solution is intended for use in cell-mediated DELFIA cytotoxicity assay. It forms part of the AD0116 DELFIA® EuTDA Cytotoxicity Reagents pack, but is also available as an individual product. The individually available DELFIA Eu-solution is sufficient for 10x96 wells.

DELFIA cytotoxicity assays offer a non-radioactive method to be used in cell mediated cytotoxicity studies. The method is based on loading cells with an acetoxymethyl ester of a fluorescence enhancing ligand. After the ligand has penetrated the cell membrane the ester bonds are hydrolyzed within the cell to form a hydrophilic ligand, which no longer passes through the membrane. After cytolysis the released ligand is introduced to a europium solution to form a fluorescent chelate. The measured signal correlates directly with the amount of lysed cells.

Specifications

Automation Compatible Yes
Detection Method DELFIA TRF, Time-Resolved Fluorescence (TRF)
Experimental Type In vitro
Product Brand Name DELFIA
Unit Size 200 mL

Tools

Resources, Events & More

Resource Type File Name File Format
Application Note DELFIA Cytotoxicity PDF 497 KB
Application Note DELFIA VICTOR Immunoassays- How to Optimize Rapid and Simple Immunoassays PDF 406 KB