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LANCE Ultra Europium-anti-phospho-ATF-2 (Thr53) Antibody, 100 µg

Rabbit monoclonal antibody labeled with the LANCE® Europium W-1024-ITC chelate. This antibody detects human Activating Transcription Factor 2 (ATF-2; Swiss-Prot entry P15336) phosphorylated at Thr53. Thr53 of ATF-2 is phosphorylated by JNK and p38 kinases. Sequence motif recognized: VADQTPTPTRFLK

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Product Number Unit Size List Price Your Price Quantity
TRF0212-D 10 µg 1926.00 USD
TRF0212-M 100 µg 6600.00 USD

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Overview

LANCE Ultra time-resolved fluorescence resonance energy transfer (TR-FRET) assays use a proprietary europium chelate donor dye, W1024 (Eu), together with ULight™, a small molecular weight acceptor dye with a red-shifted fluorescent emission. Validated for over 310 kinases, we provide data transparency by supplying:

 

 

  • Test conditions: Rapid assay setup to move straight into optimized assay performance

  • S:B data: Confidence in proven performance of the kinase on specific substrates; no guessing

  • Phospho-motifs: Substrate sequences are provided

  • Assay options: Multiple substrates are tested so you can select the best peptide sequence and S:B for your kinase

 

 

 

For this antibody:

 

 

 

  • 10 μg = 1,562 assay points

  • 100 μg = 15,625 assay points

 

 

 

In this kinase assay configuration, the phosphorylated GST-tag fusion substrate is detected by both the ULight-labeled anti-GST and Eu-labeled anti-phosphosubstrate antibodies, which brings donor and acceptor dyes into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight substrate phosphorylation.

 

Specifications

Antibody Conjugates Anti-phospho-ATF-2
Automation Compatible Yes
Detection Method TR-FRET, Time-Resolved Fluorescence (TRF)
Experimental Type In vitro
Fluorophore Eu-W1024
Molecular Modification Phosphorylation
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Unit Size 100 µg