Europium-labeled monoclonal antibody recognizing human Histone H3 that is unmodified at lysine 9 and lysine 27 (H3K9/K27). For use in LANCE® Ultra epigenetic writer and eraser assays.
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|Product Number||Unit Size||List Price||Your Price||Quantity|
|TRF0411-D||10 µg||3383.00 USD|
|TRF0411-M||100 µg||8500.00 USD|
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The LANCE® Ultra Europium-anti-unmodified Histone H3 Lysine 9/Lysine 27 (H3K9/K27) antibody was used for the development and optimization of epigenetic writer and eraser assays.
Proven, cost-efficient LANCE Ultra reagents can be used to quantitate peptide modifications, detecting specific meythylation and acetylation states. No wash, homogenous LANCE Ultra reagents are HTS friendly - design your own epigenetics screening strategy for greatest efficiency.
Epigenetic enzymatic assays are optimized using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody (Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecules into close proximity. Upon irradiation at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of biotinylated substrate modification.
|Detection Method||TR-FRET, Time-Resolved Fluorescence (TRF)|
|Experimental Type||In vitro|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Dry Ice|
|Unit Size||10 µg|
|Resource Type||File Name||File Format|
|Brochure||EPIGENETICS DRUG DISCOVERY SOLUTIONS||PDF 666 KB|
|Technical Note||LANCE Ultra HDAC1 Histone H3-Lysine 9 Deacetylase Assay||PDF 678 KB|
|Technical Note||LANCE Ultra JMJD1A Histone H3-Lysine 9 Demethylase Assay||PDF 734 KB|