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LANCE Ultra Europium-anti-tri-methyl-Histone H3 Lysine 27 (H3K27me3) Antibody, 10 µg

Europium-labeled monoclonal antibody recognizing human Histone H3 tri-methylated at lysine 27 (H3K27me3). For use in LANCE® Ultra epigenetic writer and eraser assays.

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Product Number Unit Size List Price Your Price Quantity
TRF0407-D 10 µg 3284.00 USD
TRF0407-M 100 µg 8200.00 USD

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Details

The LANCE® Ultra Europium-anti-tri-methyl-Histone H3 Lysine 27 (H3K27me3) antibody was used for the development and optimization of methyltransferase assays.

Formats:

  • 10 µg = 1,500 assay points (384-well assay format)
  • 100 µg = 15,000 assay points (384-well format)

Proven, cost-efficient LANCE Ultra reagents can be used to quantitate peptide modifications, detecting specific meythylation and acetylation states. No wash, homogenous LANCE Ultra reagents are HTS friendly - design your own epigenetics screening strategy for greatest efficiency.


Epigenetic enzymatic assays are optimized using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody (Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecules into close proximity. Upon irradiation at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of biotinylated substrate modification.

Antibody Conjugates Anti-H3K27me3
Automation Compatible Yes
Detection Method TR-FRET, Time-Resolved Fluorescence (TRF)
Experimental Type In vitro
Fluorophore Eu-W1024
Molecular Modification Methylation
One Unit Contains 10 µg
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Unit Size 10 µg

Resource Library

Resource Type File Name File Format
Technical Note Lance Ultra p300 Histone H3-Lysine Acetyltransferase Assay PDF 699 KB