PerkinElmer
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LANCE Ultra Europium-anti-phospho-PKC (Ala25Ser) peptide Antibody, 10 µg

Mouse monoclonal antibody labeled with the LANCE® Europium chelate. This antibody detects phospho-Ser in a synthetic peptide (RFARKGSLRQKNV) only after the peptide is phosphorylated by PKC or PKA kinases. The synthetic peptide is derived from amino acids 19-31 of human PKCα in which Ala25 is mutated to Ser. Sequence motif recognized: RFARKG-pS-LRQKNV

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Part Number Unit Size List Price Your Price Quantity
TRF0207-D 10 µg 1984.00 USD
TRF0207-M 100 µg 6800.00 USD
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Overview

LANCE Ultra TR-FRET technology provides a two-component kinase assay that combines direct labeling of the kinase substrate with ULight™, an innovative emission dye for optimum binding efficiency, reducing steps and assay prep time. Validated for over 310 kinases, we provide data transparency by supplying:


  • Test conditions: Rapid assay setup to move straight into optimized assay performance

  • S:B data: Confidence in proven performance of the kinase on specific substrates; no guessing

  • Phospho-motifs: Substrate sequences are provided

  • Assay options: Multiple substrates are tested so you can select the best peptide sequence and S:B for your kinase

For this antibody


  • 10 μg = 1,562 assay points

  • 100 μg = 15,625 assay points

In LANCE Ultra kinase assays, the binding of a Eu-labeled anti-phosphosubstrate antibody to the phosphorylated ULight-labeled substrate brings donor and acceptor molecules into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye, which in turn generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight-substrate phosphorylation.

Specifications

Antibody Conjugates Anti-phospho-PKC
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Fluorophore Eu-W1024
Molecular Modification Phosphorylation
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Unit Size 10 µg

Resources, Events & More

All (7)
Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Application Note Comparison of LANCE Ultra TR-FRET to PerkinElmer's Classical LANCE TR-FRET Platform for Kinase Applications PDF  805 KB
Application Note Development and Optimization of Kinase Assays using New LANCE Ultra TR-FRET Reagents PDF  583 KB
Application Note Improved Stability of the LANCE Ultra Signal in Kinase Assays PDF  206 KB
Technical Note LANCE Ultra PKC Kinase Assay PDF  480 KB
Poster Novel Substrates for Fluorescence-based Protein Tyrosine Kinase Assays PDF  276 KB
Poster Poster LANCE Ultra Tyrosine kinases PDF  276 KB