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LANCE Ultra Europium-anti-phospho-MAP Kinase p38-alpha (Thr180/Tyr182), 10 µg

Europium-labeled mouse monoclonal antibody recognizing human MAP kinase p38-alpha phosphorylated at Thr180 and Tyr182. Sequence motif recognized: DEM(pT)G(pY)VATRW

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Product Number Unit Size List Price Your Price Quantity
TRF0219-D 10 µg 1926.00 USD
TRF0219-M 100 µg 6700.00 USD

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Overview

LANCE Ultra time-resolved fluorescence resonance energy transfer (TR-FRET) assays use a proprietary europium chelate donor dye, W1024 (Eu), together with ULight™, a small molecular weight acceptor dye with a red-shifted fluorescent emission. Validated for over 310 kinases, we provide data transparency by supplying:


  • Test conditions: Rapid assay setup to move straight into optimized assay performance

  • S:B data: Confidence in proven performance of the kinase on specific substrates; no guessing

  • Phospho-motifs: Substrate sequences are provided

  • Assay options: Multiple substrates are tested so you can select the best peptide sequence and S:B for your kinase

For this antibody:


  • 10 μg = 1,562 assay points

  • 100 μg = 15,625 assay points

In this kinase assay configuration, the phosphorylated GST-tag fusion substrate is detected by both the ULight-labeled anti-GST and Eu-labeled anti-phosphosubstrate antibodies, which brings donor and acceptor dyes into close proximity. After irradiation of the kinase reaction at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of ULight substrate phosphorylation.

Specifications

Antibody Conjugates Anti-phospho-p38a
Automation Compatible Yes
Detection Method TR-FRET, Time-Resolved Fluorescence (TRF)
Experimental Type In vitro
Fluorophore Eu-W1024
Molecular Modification Phosphorylation
Product Brand Name LANCE Ultra
Shipping Condition Dry Ice
Unit Size 10 µg

Resources, Events & More

Resource Type File Name File Format
Application Note Improved Stability of the LANCE Ultra Signal in Kinase Assays PDF 206 KB
Technical Note MAP2K6 Kinase Assay ULight™ -anti-GST & Europium-anti-phospho-MAP Kinase p38-alpha (Thr180/Tyr182) Antibody PDF 1 MB