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LANCE Ultra Europium-anti-methyl-Histone H3 Lysine 9 (H3K9me2), 10 µg

Europium-labeled monoclonal antibody recognizing human Histone H3 di-methylated at lysine 9 (H3K9me2). For use in LANCE® Ultra epigenetic writer and eraser assays.

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Product Number Unit Size List Price Your Price Quantity
TRF0403-D 10 µg 3284.00 USD
TRF0403-M 100 µg 8200.00 USD

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Overview

The LANCE® Ultra Europium-anti-methyl-Histone H3 Lysine 9 (H3K9me2) antibody was used for the development and optimization of a G9a histone H3 methyltransferase assay using a
biotinylated Histone H3 (1-21) peptide as substrate. A technical note describing the assay is available in our product literature.

Formats:

  • 10 µg = 1,500 assay points (384-well assay format)
  • 100 µg = 15,000 assay points (384-well format)

Proven, cost-efficient LANCE Ultra reagents can be used to quantitate peptide modifications, detecting specific meythylation and acetylation states. No wash, homogenous LANCE Ultra reagents are HTS friendly - design your own epigenetics screening strategy for greatest efficiency.


Epigenetic enzymatic assays are optimized using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody (Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecules into close proximity. Upon irradiation at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of biotinylated substrate modification.

Specifications

Antibody Conjugates Anti-H3K9me2
Automation Compatible Yes
Detection Method TR-FRET, Time-Resolved Fluorescence (TRF)
Experimental Type In vitro
Fluorophore Eu-W1024
Molecular Modification Methylation
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Unit Size 10 µg