Europium-labeled monoclonal antibody recognizing human Histone
H3 that is unmodified at lysine 4 (H3K4). For use in LANCE® Ultra epigenetic writer and eraser assays.
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|Product Number||Unit Size||List Price||Your Price||Quantity|
|TRF0404-D||10 µg||3383.00 USD|
|TRF0404-M||100 µg||8500.00 USD|
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The LANCE® Ultra Europium-anti-unmodified Histone H3 Lysine 4 (H3K4) antibody was used for the development and optimization of a SIRT1 deacetylation assay using a biotinylated Histone H3 (1-21) peptide acetylated at lysine 4 as substrate. A technical note describing the assay is available in our product literature.
Proven, cost-efficient LANCE Ultra reagents can be used to quantitate peptide modifications, detecting specific meythylation and acetylation states. No wash, homogenous LANCE Ultra reagents are HTS friendly - design your own epigenetics screening strategy for greatest efficiency.
Epigenetic enzymatic assays are optimized using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody (Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecules into close proximity. Upon irradiation at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of biotinylated substrate modification.
|Detection Method||TR-FRET, Time-Resolved Fluorescence (TRF)|
|Experimental Type||In vitro|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Dry Ice|
|Unit Size||100 µg|
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|Resource Type||File Name||File Format|
|Technical Note||LANCE Ultra LSD1 Histone H3-Lysine 4 Demethylase Assay||PDF 485 KB|
|Technical Note||LANCE Ultra SIRT1 Histone H3-Lysine 4 Deacetylase Assay||PDF 464 KB|
|Technical Note||Lance Ultra p300 Histone H3-Lysine Acetyltransferase Assay||PDF 699 KB|
|Poster||Development of High-Throughput Assays to Study Histone H3K4 Methyltransferases & H3K9 Methyl- and Acetyltransferases||PDF 386 KB|
|Poster||Development of High-Throughput Assays to Study Methylases, Demethylases and Deacetylases Targeting Histone H3K4, H3K27 and H3K36 Residues||PDF 359 KB|
|Poster||Development of Homogeneous Non-radioactive Assays for Studying Histone H3 Methyltransferases and Demethylases||PDF 381 KB|
|Poster||Development of Homogeneous Proximity Assays for JMJD2A/2C Histone Demethylases||PDF 289 KB|
|Poster||Development of a Non-Radioactive, No-Wash Biochemical Assay for High-Throughput Screening of Small Molecule Modulators of DNA MethyltransferasesNathalie||PDF 386 KB|