Europium-labeled monoclonal antibody recognizing human Histone H3 acetylated at lysine 9 (H3K9ac). For use in LANCE® Ultra epigenetic writer and eraser assays.
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|Product Number||Unit Size||List Price||Your Price||Quantity|
|TRF0400-D||10 µg||3284.00 USD|
|TRF0400-M||100 µg||8200.00 USD|
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The LANCE® Ultra Europium-anti-acetyl-Histone H3 Lysine 9 (H3K9ac) antibody was used for the development and optimization of a p300 acetyltransferase assay using a biotinylated Histone H3 (1-21) peptide as substrate. A technical note describing the assay is available in our product literature.
Proven, cost-efficient LANCE Ultra reagents can be used to quantitate peptide modifications, detecting specific meythylation and acetylation states. No wash, homogenous LANCE Ultra reagents are HTS friendly - design your own epigenetics screening strategy for greatest efficiency.
Epigenetic enzymatic assays are optimized using a biotinylated histone H3-derived peptide as substrate. The modified peptide is captured by the Eu-labeled antibody (Ab) and ULight-Streptavidin (SA) which bring the Eu donor and ULight acceptor dye molecules into close proximity. Upon irradiation at 320 or 340 nm, the energy from the Eu donor is transferred to the ULight acceptor dye which, in turn, generates light at 665 nm. The intensity of the light emission is proportional to the level of biotinylated substrate modification.
|Detection Method||TR-FRET, Time-Resolved Fluorescence (TRF)|
|Experimental Type||In vitro|
|Product Brand Name||LANCE Ultra|
|Shipping Condition||Blue Ice|
|Unit Size||100 µg|
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|Resource Type||File Name||File Format|
|Technical Note||Lance Ultra p300 Histone H3-Lysine Acetyltransferase Assay||PDF 699 KB|
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|Poster||Development of Homogeneous Non-radioactive Assays for Studying Histone H3 Methyltransferases and Demethylases||PDF 381 KB|
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|Poster||Development of a Non-Radioactive, No-Wash Biochemical Assay for High-Throughput Screening of Small Molecule Modulators of DNA MethyltransferasesNathalie||PDF 386 KB|