Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
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This dinitrophenol (DNP) labeled analog of uridine triphosphate (UTP) is used for labeling RNA probes via in vitro transcription with various RNA polymerases. DNP labeled RNA probes are used in a variety of molecular biology applications including in situ hybridization and gene expression microarrays. DNP-labeled products may be detected with anti-DNP antibody conjugates. For the highest sensitivity in detection, we recommend using a anti-DNP-HRP conjugate with tyramide signal amplification (TSA).
The number “11” in the compound name refers to the length of the linker arm between UTP and the label.
|One Unit Contains||250 nmol|
|Resource Type||File Name||File Format|
|Application Note||Signal Amplification Strategies for Rapid and Sensitive High Content Analysis||PDF 776 KB|
|Data Sheet||NEL555 - DNP-11-UTP - Technical Data Sheet||PDF 49 KB|