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DELFIA® Streptavidin-coated yellow plate, 96-well, 10 plates

96-well yellow microplates coated with streptavidin, for biotinylated biomolecules for use in DELFIA time-resolved fluorescence assays.

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Product Number List Price Your Price Quantity
AAAND-0005 455.00 USD

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Streptavidin-coated DELFIA® plates bind all biotinylated biomolecules; e.g. peptides, proteins, antibodies, and haptens. The biotin binding capacity is 10 pmol/well in 200 µL volume. DELFIA® Yellow plates have an exceptionally low fluorescence background.

DELFIA® (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of a compound or biomolecule using lanthanide chelate labeled reagents, separating unbound reagent using wash steps. DELFIA assays are flexible, compatible with a variety of plate readers, and, as this is a wash-based technology, compatible with most sample types. The technology is based on fluorescence of lanthanide chelates (Europium, Samarium, and Terbium). The fluorescence decay time of these lanthanide chelate labels is much longer than traditional fluorophores, allowing efficient use of temporal resolution for reduction of autofluorescent background. The large Stokes’ shift (difference between excitation and emission wavelengths) and the narrow emission peaks contribute to increasing signal-to-noise ratio. Sensitivity is further increased because of the dissociation-enhancement principle: the lanthanide chelate is dissociated and a new highly fluorescent chelate is formed into a protective micellar solution. DELFIA lanthanide chelates require this dissociation/enhancement step for fluorescence (induced by addition of DELFIA Enhancement solution, DELFIA Inducer, and DELFIA Enhancer as appropriate to the particular lanthanide chelate).

Automation Compatible Yes
Coating Treatment Streptavidin
Color Yellow
Detection Method DELFIA TRF, Time-Resolved Fluorescence (TRF)
Material Polystyrene
One Unit Contains 10 plates
Product Brand Name DELFIA
Shipping Condition Ambient
Unit Size Pack of 10
Wells Number 96 well plate


Resource Library & Events

Resource Type File Name File Format
Application Note Application Notes - Delfia Protein Kinase Assays PDF 461 KB
Application Note DELFIA VICTOR Immunoassays- How to Optimize Rapid and Simple Immunoassays PDF 406 KB
Application Note Multiplexing DELFIA® assays using lanthanide-labeled probes PDF 121 KB