Buffer for DELFIA® time-resolved fluorescence (TRF) ligand binding assays.
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DELFIA® L*R binding buffer concentrate (10x) is a buffered protein, salt and detergent solution intended for use in dissociation-enhanced time-resolved fluorometric ligand receptor binding assays (DELFIA®). This is a basic ligand receptor binding buffer, which can be supplemented with receptor-specific reagents. It is optimized to give a minimum fluorescence background and non-specific binding in DELFIA® ligand receptor binding assays.
DELFIA L*R binding buffer concentrate (10x) is a 10-fold concentrate containing 500 mmol/L Tris-HCI buffered salt solution (pH 7.5) with 50 mmol/L MgCl2, 250 µmol/L ethylenediaminetetraacetic acid (EDTA), 2 % bovine serum albumin (BSA), and 0.05 % sodium azide (NaN3) as preservative.
|Detection Method||Time-Resolved Fluorescence (TRF), DELFIA TRF|
|Experimental Type||In vitro|
|Product Brand Name||DELFIA|
|Shipping Condition||Blue Ice|
|Unit Size||250 mL|