DELFIA® Inducer is used to create a fluorescent Europium or Samarium chelate for measuring DELFIA time-resolved fluorescence (TRF) assays. This buffer is an alternative to Enhancement Solution for Eu and/or Sm measurements.
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DELFIA® Inducer is a ready-for-use solution with Triton X-100, glycine, hydrochloric acid and chelators. It is intended for use in the rapid quantitative determination of Eu3+ and Sm3+ in time-resolved fluorometric assays. It is suitable for use in multilabel assays that require DELFIA® Enhancer for development of Tb3+ and Dy3+ fluorescence. The solution dissociates lanthanide from solid phase bound DELFIA® chelate labeled biomolecules in a few minutes depending from used chelate to form a homogeneous and highly fluorescent Eu/Sm-(BFPP)3(TOPO)2-3 micellar chelate solution. The solution allows highly sensitive Eu3+/Sm3+ measurements to be performed when using a time-resolved fluorometer.
For use with stable chelates such as DTPA, to provide more rapid signal development. Suitable for use in all multianalyte assays.
|Detection Method||Time-Resolved Fluorescence (TRF), DELFIA TRF|
|Experimental Type||In vitro|
|Product Brand Name||DELFIA|
|Unit Size||250 mL|
The DELFIA Inducer is an acidic chealting detergent solution intended for use in the rapid quantitative determination of Eu3+/Sm3+ in time-resolved fluoroimmunoassays.