PerkinElmer
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DELFIA Eu-N1 ITC chelate & Eu standard, 1 mg

DELFIA® Europium labeling chelate and Europium standard for labeling your own biomolecules for use in DELFIA time-resolved fluorescence assays. The ITC chelate reacts with free amine groups in the target biomolecule.

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1244-301
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Detail Information

The DELFIA® Eu-N1 ITC chelate is optimized for the europium labeling of proteins and peptides for use in dissociation-enhanced time-resolved fluorometric assays. Isothiocyanate (ITC) activated chelates react primarily with free amine groups such as those at the N-terminus of protein or lysine residues. 1 mg allows labeling of up to 5 mg of antibody. Europium standard is provided to determine labeling ratio and use as an instrument control.

Specifications

Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), DELFIA TRF
Experimental Type In vitro
Fluorophore Eu-N1-ITC
Product Brand Name DELFIA
Shipping Condition Blue Ice
Unit Size 1 mg
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Application Note

DELFIA Assays: Flexible and Sensitive Tools for Monoclonal Antibody Development

DELFIA® immunoassays are particularly well suited for discovery of specific, high-affinity monoclonal antibodies (mAbs), especially at the low concentrations that may be present in hybridoma supernatants. An assay used in these conditions must be sensitive, specific, reliable, reproducible and easy enough to handle 1,000 to 1,500 samples in one batch.

PDF 610 KB
DELFIA® assays bring convenience in monoclonal anitbody development

The DELFIA® system provides a method of discovering specific, high-affinity monoclonal antibodies (Mabs) even in low concentrations in hybridoma supernatants. The assay used in these, conditions has to be very sensitive, specific, reliable, reproducible and easy enough to allow handling of about 1,000 to 1,500 samples in one batch.

PDF 464 KB

Manual

DELFIA Eu-Labelling Reagent 1244-301

DELFIA Eu-Labeling Reagent is optimized for the europium labeling of proteins and peptides.

PDF 86 KB