Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The amino-activated chelate reacts with free carboxyl groups.
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DELFIA® Euporium Chelate of N1-(p-iodoacetamindobenzyl)diethylenetriamine-N1, N2, N3, N3-tetraacetic acid is optimized for labeling of small compounds containing at least one carboxyl group. The labeling compound can be used in dissociation-enhanced time-resolved fluorometric assays.
The N1 chelates can be used in DELFIA® separation assays where the concentration of chelating agents is <0.05 mM, pH >7, and the temperature is < 38°C. They are suitable for highly sensitive end point measurements in separation based assays such those for detection of protein-protein binding and cell adhesion as well as for immunoassays. Amino activated chelates react with carboxyl groups on the compound to be labeled. The reagent concentration should be equimolar to the carboxylate group.
DELFIA® (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of a compound or biomolecule using lanthanide chelate labeled reagents, separating unbound reagent using wash steps. DELFIA assays are flexible, compatible with a variety of plate readers, and, as this is a wash-based technology, compatible with most sample types. The technology is based on fluorescence of lanthanide chelates (Europium, Samarium, and Terbium). The fluorescence decay time of these lanthanide chelate labels is much longer than traditional fluorophores, allowing efficient use of temporal resolution for reduction of autofluorescent background. The large Stokes’ shift (difference between excitation and emission wavelengths) and the narrow emission peaks contribute to increasing signal-to-noise ratio. Sensitivity is further increased because of the dissociation-enhancement principle: the lanthanide chelate is dissociated and a new highly fluorescent chelate is formed into a protective micellar solution. DELFIA lanthanide chelates require this dissociation/enhancement step for fluorescence (induced by addition of DELFIA Enhancement solution, DELFIA Inducer, and DELFIA Enhancer as appropriate to the particular lanthanide chelate).
|Detection Method||Time-Resolved Fluorescence (TRF), DELFIA TRF|
|Experimental Type||In vitro|
|Product Brand Name||DELFIA|
|Shipping Condition||Blue Ice|
|Unit Size||1 mg|
|Resource Type||File Name||File Format|
|Application Note||Multiplexing DELFIA® assays using lanthanide-labeled probes||PDF 121 KB|
|Application Note||Simple Conversion of ELISA to DELFIA TRF Technology||PDF 1 MB|
|Manual||DELFIA Eu-N1 Amino Chelate & Europium Standard AD0003||PDF 87 KB|