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DELFIA Eu-DTPA-ITC Chelate and Europium (Eu) Standard, 1mg

Europium labeling chelate for creating your own Europium-labeled biomolecules for use in DELFIA time-resolved fluorescence (TRF) assays. The ITC-activated chelate reacts with free amino groups, such as lysines. The DTPA chelate is more-stable than the N1 chelate.

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Overview

DELFIA® Eu-DTPA ITC Chelate is optimized for the europium labeling of proteins and peptides for use in dissociation-enhanced time resolved fluorometric assays, where especially high chelate stability is required. It is recommended for assays where chelate has to be stable in in vivo conditions (e.g. pharmacokinetics) or assays where high concentrations of competing chelating agents may be present. The reagent is the Eu3+-chelate of N1-(p-iodoacetamindobenzyl)diethylenetriamine-N1,N2, N3, N3-tetraacetic acid. The isothiocyanato group reacts with free amino groups on the proteins and peptides, forming stable, covalent thiourea bond.

The DTPA-chelates can be used in DELFIA® separation assays where: the concentration of chelating agents is <10 mM, pH>6.5, and the assay temperature is <70°C. They can stand 2 to 3 mmol EDTA when pH is 7.5 or higher. The enhancement time using a DELFIA® shaker is 25 minutes. Isothiocyanate (ITC) activated chelates, react primarily with free lysines on the compound to be labeled.

DELFIA® (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of a compound or biomolecule using lanthanide chelate labeled reagents, separating unbound reagent using wash steps. DELFIA assays are flexible, compatible with a variety of plate readers, and, as this is a wash-based technology, compatible with most sample types. The technology is based on fluorescence of lanthanide chelates (Europium, Samarium, and Terbium). The fluorescence decay time of these lanthanide chelate labels is much longer than traditional fluorophores, allowing efficient use of temporal resolution for reduction of autofluorescent background. The large Stokes’ shift (difference between excitation and emission wavelengths) and the narrow emission peaks contribute to increasing signal-to-noise ratio. Sensitivity is further increased because of the dissociation-enhancement principle: the lanthanide chelate is dissociated and a new highly fluorescent chelate is formed into a protective micellar solution. DELFIA lanthanide chelates require this dissociation/enhancement step for fluorescence (induced by addition of DELFIA Enhancement solution, DELFIA Inducer, and DELFIA Enhancer as appropriate to the particular lanthanide chelate).

Specifications

Automation Compatible Yes
Detection Method DELFIA TRF, Time-Resolved Fluorescence (TRF)
Experimental Type In vitro
Fluorophore Eu-DTPA-ITC
Product Brand Name DELFIA
Shipping Condition Blue Ice
Unit Size 1 mg

Resources, Events & More

Resource Type File Name File Format
Application Note DELFIA VICTOR Immunoassays- How to Optimize Rapid and Simple Immunoassays PDF 406 KB
Application Note Multiplexing DELFIA® assays using lanthanide-labeled probes PDF 121 KB
Manual DELFIA Eu-DTPA ITC Chelate & Europium Standard PDF 88 KB