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DELFIA Eu-N1 Anti-Phosphotyrosine (PY20), 50µg

Europium-labeled anti-phosphotyrosine (PY20) antibody, for use in DELFIA time-resolved fluorescence (TRF) kinase assays, phosphoprotein detection, and phosphoprotein binding assays.

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Product Number Unit Size List Price Your Price Quantity
AD0038 50 µg 243.00 USD
AD0039 1 mg 3063.00 USD

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Overview

Generic DELFIA® reagents are intended for use in dissociation-enhanced time-resolved fluorometric assays. All reagents have been labeled using the DELFIA® N1-chelate. They are suitable for highly sensitive end point measurements in separation based assays such as those for detection of protein-protein binding and cell adhesion as well as for immunoassays.

The PY20 antibody (IgG2b) and PT66 antibody are mouse monoclonal antibodies that bind to phosphorylated tyrosine residues.

DELFIA® (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of a compound or biomolecule using lanthanide chelate labeled reagents, separating unbound reagent using wash steps. DELFIA assays are flexible, compatible with a variety of plate readers, and, as this is a wash-based technology, compatible with most sample types. The technology is based on fluorescence of lanthanide chelates (Europium, Samarium, and Terbium). The fluorescence decay time of these lanthanide chelate labels is much longer than traditional fluorophores, allowing efficient use of temporal resolution for reduction of autofluorescent background. The large Stokes’ shift (difference between excitation and emission wavelengths) and the narrow emission peaks contribute to increasing signal-to-noise ratio. Sensitivity is further increased because of the dissociation-enhancement principle: the lanthanide chelate is dissociated and a new highly fluorescent chelate is formed into a protective micellar solution. DELFIA lanthanide chelates require this dissociation/enhancement step for fluorescence (induced by addition of DELFIA Enhancement solution, DELFIA Inducer, and DELFIA Enhancer as appropriate to the particular lanthanide chelate).

Specifications

Antibody Conjugates PY20
Automation Compatible Yes
Detection Method DELFIA TRF, Time-Resolved Fluorescence (TRF)
Experimental Type In vitro
Fluorophore Eu-N1
Molecular Modification Phosphorylation
Product Brand Name DELFIA
Shipping Condition Dry Ice
Unit Size 50 µg

Resources, Events & More

Resource Type File Name File Format
Application Note Application Notes - Delfia Protein Kinase Assays PDF 461 KB
Application Note DELFIA VICTOR Immunoassays- How to Optimize Rapid and Simple Immunoassays PDF 406 KB
Manual Generic DELFIA Reagents PDF 90 KB