Europium-labeled anti-6X His antibody, for capturing His-tagged proteins in DELFIA® TRF assays. The anti-6xHis antibody is a purified mouse IgG1 monoclonal antibody. It is raised against a polypeptide containing a 6x histidine tag. The antibody has been shown to detect polyhistidine tags localized at the amino- or carboxyl-terminus.
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Generic DELFIA® reagents are intended for use in dissociation-enhanced time-resolved fluorometric assays. All reagents have been labeled using the DELFIA® N1-chelate. They are suitable for highly sensitive end point measurements in separation based assays such as those for detection of protein-protein binding and cell adhesion as well as for immunoassays.
DELFIA® (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of a compound or biomolecule using lanthanide chelate labeled reagents, separating unbound reagent using wash steps. DELFIA assays are flexible, compatible with a variety of plate readers, and, as this is a wash-based technology, compatible with most sample types. The technology is based on fluorescence of lanthanide chelates (Europium, Samarium, and Terbium). The fluorescence decay time of these lanthanide chelate labels is much longer than traditional fluorophores, allowing efficient use of temporal resolution for reduction of autofluorescent background. The large Stokes’ shift (difference between excitation and emission wavelengths) and the narrow emission peaks contribute to increasing signal-to-noise ratio. Sensitivity is further increased because of the dissociation-enhancement principle: the lanthanide chelate is dissociated and a new highly fluorescent chelate is formed into a protective micellar solution. DELFIA lanthanide chelates require this dissociation/enhancement step for fluorescence (induced by addition of DELFIA Enhancement solution, DELFIA Inducer, and DELFIA Enhancer as appropriate to the particular lanthanide chelate).
|Antibody Conjugates||Anti-6X His|
|Detection Method||Time-Resolved Fluorescence (TRF), DELFIA TRF|
|Experimental Type||In vitro|
|Product Brand Name||DELFIA|
|Shipping Condition||Dry Ice|
|Unit Size||50 µg|
Technical Information, Kit Inserts, AD0038, AD0039, AD0040, AD0041, AD0046, AD0047, AD0048, AD0049, AD0050, AD0053, AD0054, AD0105, AD0106, AD0108, AD0109, AD0112, AD0113, AD0124, AD0159, AD0160, AD0207, AD0250, AD0251