96-well yellow microplates coated with anti-rabbit IgG, for binding rabbit IgG antibodies for use in DELFIA time-resolved fluorescence assays.
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Anti-rabbit antibody coated DELFIA® plates are based
on an anti-rabbit antibody raised in goat.
DELFIA® Yellow plates have an exceptionally low fluorescence background.
DELFIA® (dissociation-enhanced lanthanide fluorescence immunoassay) is a time-resolved fluorescence (TRF) intensity technology. Assays are designed to detect the presence of a compound or biomolecule using lanthanide chelate labeled reagents, separating unbound reagent using wash steps. DELFIA assays are flexible, compatible with a variety of plate readers, and, as this is a wash-based technology, compatible with most sample types. The technology is based on fluorescence of lanthanide chelates (Europium, Samarium, and Terbium). The fluorescence decay time of these lanthanide chelate labels is much longer than traditional fluorophores, allowing efficient use of temporal resolution for reduction of autofluorescent background. The large Stokes’ shift (difference between excitation and emission wavelengths) and the narrow emission peaks contribute to increasing signal-to-noise ratio. Sensitivity is further increased because of the dissociation-enhancement principle: the lanthanide chelate is dissociated and a new highly fluorescent chelate is formed into a protective micellar solution. DELFIA lanthanide chelates require this dissociation/enhancement step for fluorescence (induced by addition of DELFIA Enhancement solution, DELFIA Inducer, and DELFIA Enhancer as appropriate to the particular lanthanide chelate).
|Coating Treatment||Anti-rabbit IgG|
|Detection Method||Time-Resolved Fluorescence (TRF), DELFIA TRF|
|Product Brand Name||DELFIA|
|Shipping Condition||Blue Ice|
|Unit Size||Pack of 10|
|Wells Number||96 well plate|
|Resource Type||File Name||File Format|
|Application Note||DELFIA VICTOR Immunoassays- How to Optimize Rapid and Simple Immunoassays||PDF 406 KB|
|Application Note||Multiplexing DELFIA® assays using lanthanide-labeled probes||PDF 121 KB|
|Application Note||Simple Conversion of ELISA to DELFIA TRF Technology||PDF 1 MB|