PerkinElmer
check quantity

CXCL10a (human) LANCE Ultra TR-FRET Detection Kit, 10,000 Assay Points

The LANCE® Ultra Human CXCL10 Detection Kit is designed for detection and quantitation of human CXCL10 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

Please enter valid quantity

Please login to add favorites

NULL OR EMPTY CART

Part Number Unit Size List Price Your Price Quantity
TRF1259C 500 Assay Points 772.00 USD
TRF1259M 10,000 Assay Points 10300.00 USD
Buy Now

You successfully added item(s) to your cart

Overview

The LANCE® Ultra Human CXCL10 (IP-10) Detection Kit is designed for detection and quantitation of human CXCL10 in cell culture media using a homogeneous TR-FRET (no-wash steps, no separation steps) assay.

  • No-wash steps, no separation steps
  • TR-FRET technology
  • Sensitive detection
  • High reproducibility
  • Faster time-to-results
  • Easy automation
  • 96-well, 384-well, and 1536-well formats

LANCE® and LANCE® (Lanthanide chelate excite) Ultra are our TR-FRET (time-resolved fluorescence resonance energy transfer), homogeneous (no wash) technologies. One antibody of interest is labeled with a donor fluorophore (a LANCE Europium chelate) and the second molecule is labeled with an acceptor fluorophore (ULight™ dye). Upon excitation at 320 or 340 nm, energy can be transferred from the donor Europium chelate to the acceptor fluorophore if sufficiently close for FRET (~10 nm). This results in the emission of light at 665 nm.

C-X-C Motif Chemokine 10 (CXCL10), also known as 10 kDa Interferon-gamma-Induced Protein (IP-10), is a 77 amino acid protein secreted by several cell types in response to IFN-γ. These cell types include monocytes, endothelial cells, and fibroblasts. CXCL10 has been attributed several roles such as chemoattraction for monocytes, T cells, and dendritic cells. Other activities include promotion of T cell adhesion to endothelial cells, antitumor activity, and inhibition of bone marrow colony formation. It has been suggested that CXCL10 may play an important role in delayed hypersensitivity reactions. Increased levels of CXCL10 are found in psoriatic plaques characterized by the infiltration of neutrophils, but it does not activate neutrophils. It was reported that CXCL10 also possesses antimicrobial activity. Recent data suggest that CXCL10 could bind to toll-like receptor 4 and may contribute to beta cell failure in diabetes.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target CXCL10
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Time-Resolved Fluorescence (TRF), TR-FRET
Experimental Type In vitro
Product Brand Name LANCE Ultra
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Inflammation
Unit Size 10,000 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure LANCE product listing PDF  77 KB
Application Note Quantifying Bcl-2 Expression Levels in Human Cells with LANCE Ultra TR-FRET PDF  3 MB
Manual LANCE Ultra Human CXCL10 (IP-10) Detection Kit TRF1259 PDF  500 KB
Flyer LANCE Ultra Biomarker Detection Kits PDF  585 KB