Our bioluminescent light-producing oncology cell lines are labeled with luciferase or dual labeled with luciferase and fluorescent reporters (GFP) for in vivo studies.
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The PC3-Red-FLuc-GFP Bioware® Brite Cell Line is a light-producing and fluorescent cell line derived from PC3 human prostate adenocarcinoma. The cells have been stably transduced with the red-shifted firefly luciferase gene from Luciola Italica (Red-FLuc) fused to GFP (Green Fluorescent Protein), for a brighter, red-shifted bioluminescent or green fluorescent signal.
This cell line has very bright bioluminescence, emitting at least 30,000 photons/cell/sec in vitro. The exact number will vary depending on imaging and culturing conditions.
By emitting intensified, longer wavelength light that is significantly brighter than other firefly luciferases, our bioluminescent oncology cell lines allow you to visualize and monitor the growth of deep tissue tumors in vivo. The GFP fusion also allows for fluorescent imaging in vivo or ex vivo.
After the cells are injected into an appropriate mouse model, the optimized Red-FLuc luciferase enables more sensitive in vivo optical detection with less tissue attenuation so you can detect tumor development earlier, and monitor tumor growth and metastases in both subcutaneous and orthotopic models.
Disclaimer: For laboratory use only. This product is intended for animal research only and not for use in humans.
|Oncology Cell Line||Prostate|
|Product Brand Name||Bioware Brite|
|Shipping Condition||Dry Ice|
|Unit Size||2 million cells|
|Resource Type||File Name||File Format|
|Brochure||In Vivo Imaging Agents Brochure||PDF 2 MB|
|Guide||Bioware Brite Cell Culture Guidelines||PDF 290 KB|
|Guide||Cell Preparation and Instructions for In Vivo Injection of Bioware® CellsBrite Tumor Cells||PDF 169 KB|
|Citations Library||View peer reviewed publications using PerkinElmer's In Vivo Imaging Reagents||Link|