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DELFIA® BATDA Labeling Reagent, 50 µL

BATDA reagents for labeling cells in DELFIA time-resolved fluroescence (TRF) assays.

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Product Number List Price Your Price Quantity
C136-100 175.00 USD

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Overview

The DELFIA® BATDA Reagent is intended for labeling of cells to be used in short term cytotoxicity tests performe utilizing time-resolved fluorometry in the detection. It forms part of the AD0116 DELFIA® EuTDA Cytotoxicity Reagents pack, but is also available as an individual product. The individually available DELFIA BATDA labeling reagent is sufficient for 10x96 wells.

DELFIA cytotoxicity assays offer a non-radioactive method to be used in cell mediated cytotoxicity studies. The method is based on loading cells with an acetoxymethyl ester of a fluorescence enhancing ligand. After the ligand has penetrated the cell membrane the ester bonds are hydrolyzed within the cell to form a hydrophilic ligand, which no longer passes through the membrane. After cytolysis the released ligand is introduced to a europium solution to form a fluorescent chelate. The measured signal correlates directly with the amount of lysed cells.

Specifications

Automation Compatible Yes
Detection Method DELFIA TRF, Time-Resolved Fluorescence (TRF)
Experimental Type In vitro
Product Brand Name DELFIA
Shipping Condition Blue Ice
Unit Size 50 µL

Resources, Events & More

Resource Type File Name File Format
Application Note DELFIA Cytotoxicity PDF 497 KB
Application Note DELFIA VICTOR Immunoassays- How to Optimize Rapid and Simple Immunoassays PDF 406 KB