BATDA reagents for labeling cells in DELFIA time-resolved fluroescence (TRF) assays.
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The DELFIA® BATDA Reagent is intended for labeling of cells to be used in short term cytotoxicity tests performe utilizing time-resolved fluorometry in the detection. It forms part of the AD0116 DELFIA® EuTDA Cytotoxicity Reagents pack, but is also available as an individual product. The individually available DELFIA BATDA labeling reagent is sufficient for 10x96 wells.
DELFIA cytotoxicity assays offer a non-radioactive method to be used in cell mediated cytotoxicity studies. The method is based on loading cells with an acetoxymethyl ester of a fluorescence enhancing ligand. After the ligand has penetrated the cell membrane the ester bonds are hydrolyzed within the cell to form a hydrophilic ligand, which no longer passes through the membrane. After cytolysis the released ligand is introduced to a europium solution to form a fluorescent chelate. The measured signal correlates directly with the amount of lysed cells.
|Detection Method||Time-Resolved Fluorescence (TRF), DELFIA TRF|
|Experimental Type||In vitro|
|Product Brand Name||DELFIA|
|Shipping Condition||Blue Ice|
|Unit Size||50 µL|
|Resource Type||File Name||File Format|
|Application Note||A Simplified, Gentle Cell-Labelling Method for Non-Radioactive Cytotoxicity Assays||PDF 497 KB|
|Application Note||DELFIA VICTOR Immunoassays- How to Optimize Rapid and Simple Immunoassays||PDF 406 KB|