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ATPlite 1step Luminescence Assay System, 100 mL Assay Kit

ATP-monitoring luminescence assay for quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells. The 1-step format only requires one addition step, and can be used for continuous processing.

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6016731 444.00 USD

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Details

ATP is a marker for cell viability because it is present in all metabolically active cells. Because ATP concentration declines rapidly when cells undergo necrosis or apoptosis, monitoring ATP is a good indicator of cytocidal, cytostatic and proliferation effects. Our ATPlite and ATPlite 1step luminescence assay systems use patented innovative technologies that measure cell proliferation and cytotoxicity in mammalian cells based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin.

Features and benefits:

  • Simple and reproducible: only one reagent addition step, no separation steps
  • Suitable for both 96- and 384-well microplates
  • Linear correlation between cell number and luminescent signal: up to 50,000 cells per well for 96-well microplates and 12,500 cells per well for 384-well microplates
  • Designed for continuous process systems: luminescence should be measured between 0 and 30 minutes after reagent addition and plate shaking
  • Homogeneous: no cell harvesting or centrifugation required
  • Highly sensitive
  • Reduced Phenol Red dependency
  • High light-output: kit can also be used with less sensitive luminescence readers like multi-label readers
  • Fast: no luminescence signal stabilization time required

Assay Protocol 1-step
Automation Compatible Yes
Detection Method Luminescence
Experimental Type In vitro
One Unit Contains 100 mL
Product Brand Name ATPlite
Shipping Condition Ambient
Unit Size 1,000 (96-well) / 4,000 (384-well)
Volume Capacity 100 mL

Resource Library

Resource Type File Name File Format
Application Note Utilization of the ATPlite 1step Detection System for Homogeneous Automated Cytotoxicity Assays PDF 299 KB
Manual ATPlite 1step manual PDF 617 KB