PerkinElmer
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ATPlite 1step Luminescence Assay System, 1,000 mL ATP Assay Kit

ATP luminescence assay for quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells. The 1step luciferase ATP assay format only requires one addition step, and can be used for continuous processing.

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Part Number Unit Size List Price Your Price Quantity
6016736 10 mL 60.00 USD
6016731 100 mL 454.00 USD
6016739 1,000 mL 3042.00 USD
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Overview

ATP is a marker for cell viability due to its presence in all metabolically active cells. ATP concentration declines rapidly when cells undergo necrosis or apoptosis, and so monitoring ATP is a good indicator of cytotoxic, cytostatic and proliferation effects.

Our ATPlite 1step ATP luminescence assays use patented technologies to measure cell proliferation and cytotoxicity in mammalian cells based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin.

Features and benefits:

  • Simple and reproducible – only one reagent addition step, no separation steps
  • Suitable for both 96- and 384-well microplates
  • Linear correlation between cell number and luminescent signal: up to 50,000 cells per well for 96-well microplates and 12,500 cells per well for 384-well microplates
  • Designed for continuous process systems: luminescence should be measured between 0 and 30 minutes after reagent addition and plate shaking
  • Homogeneous – no cell harvesting or centrifugation required
  • High sensitivity – able to detect down on one cell per well
  • Reduced Phenol Red dependency
  • High light-output – assay can be used with less sensitive luminescence readers such as multi-label readers
  • Fast – no luminescence signal stabilization time required

An alternative format of this assay is also available. The ATPlite assay has separate cell lysis and luminescent signal generation steps. This allows for a more stable signal, with half-life of at least 4 hours.

Specifications

Assay Protocol 1-step
Automation Compatible Yes
Detection Method Luminescence
Experimental Type In vitro
Product Brand Name ATPlite
Quantity in a Package Amount 10000.0 Units
Shipping Condition Ambient
Unit Size 1,000 mL

Resources, Events & More

Resource Type File Name File Format
Application Note Utilization of the ATPlite 1step Detection System for Homogeneous Automated Cytotoxicity Assays PDF  299 KB
Manual ATPlite 1step manual PDF  617 KB
Flyer Add a new dimension to your research PDF  925 KB