ATP luminescence assay for quantitative evaluation of proliferation and cytotoxicity of cultured mammalian cells. The 1step luciferase ATP assay format only requires one addition step, and can be used for continuous processing.
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ATP is a marker for cell viability due to its presence in all metabolically active cells. ATP concentration declines rapidly when cells undergo necrosis or apoptosis, and so monitoring ATP is a good indicator of cytotoxic, cytostatic and proliferation effects.
Our ATPlite 1step ATP luminescence assays use patented technologies to measure cell proliferation and cytotoxicity in mammalian cells based on the production of light caused by the reaction of ATP with added luciferase and D-luciferin.
Features and benefits:
An alternative format of this assay is also available. The ATPlite assay has separate cell lysis and luminescent signal generation steps. This allows for a more stable signal, with half-life of at least 4 hours.
|Experimental Type||In vitro|
|Product Brand Name||ATPlite|
|Unit Size||1,000 mL|
A number of strategies are currently being implemented in an effort to increase the success rate in identifying potential therapeutic lead compounds and reducing the number of late stage clinical trial failures. One approach is to perform ADME/Tox profiling at an earlier stage in the drug discovery process.