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Anti-6X His AlphaPlex-545 (Tb) Acceptor beads, 250 µg

AlphaPlex-545 Acceptor beads conjugated to anti-6XHis antibody, for use in Alpha no-wash multiplexing assays.

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Part Number Unit Size List Price Your Price Quantity
AP128TB-C 250 µg 570.00 USD
AP128TB-M 5 mg 5700.00 USD
AP128TB-R 25 mg 23400.00 USD
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Overview

AlphaPlex-545 Acceptor beads conjugated to anti-6XHis antibody. The mouse monoclonal antibody detects proteins containing accessible consecutive histidine residues at the amino or carboxy terminus. These beads can be used to capture His-tagged proteins and peptides, and can be used with Alpha Donor beads to create AlphaPlex no-wash assays for:

  • Protein-protein interaction assays
  • Protein detection assays
  • Enzymatic assays

AlphaPlex Acceptor beads are intended to be used in multiplexing assays, in conjunction with AlphaLISA Acceptor beads and Alpha Donor beads. In a typical Alpha assay:

  • 250 µg of Acceptor beads is sufficient to run 250-500 wells using a 50 µL reaction volume
  • 5 mg of Acceptor beads is sufficient to run 5,000-10,000 wells using a 50 µL reaction volume
  • 25 mg of Acceptor beads is sufficient to run 25,000-50,000 wells using a 50 µL reaction volume

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Antibody Conjugates Anti-6X His
Automation Compatible Yes
Bead Type or Core Bead Type AlphaPlex Terbium (545) Acceptor
Detection Method Alpha
Product Brand Name AlphaPlex
Shipping Condition Blue Ice
Unit Size 250 µg

Resources, Events & More

All (7)
Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Guide Alpha Protein-Protein Interaction Quick Start Guide PDF  380 KB
Guide AlphaPlex Assay Development Guide 2015 PDF  1 MB
Guide AlphaPlex Quick Start Guide PDF  431 KB
Guide User's Guide To Alpha Assays Protein:Protein Interactions PDF  3 MB
Application Note Development of Pharmacokinetic (PK) Assays for Detecting Biosimilars Targeting TNFa Using AlphaLISA PDF  2 MB
Event Society of Laboratory Automation & Screening (SLAS) Event