Hapten-labeled nucleotide analogs for enzymatic incorporation, for indirect detection.
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Aminodigoxigenin (DIG) labeled labeled dideoxyguanosine triphosphate is biologically active and can be incorporated with a variety of DNA polymerases including Taq, Klenow fragment, and Sequenase™. It terminates further incorporation and enables identification of the complementary base for applications like DNA sequencing and detection of point mutations. Aminodigoxigenin-labeled products may be detected with anti-DIG antibody conjugates. For the highest sensitivity in detection, we recommend using an anti-DIG-HRP conjugate with tyramide signal amplification (TSA).
The number "9" in the compound name refers to the length of the linker arm between dCTP and the label.
|One Unit Contains||25 nmol|
|Resource Type||File Name||File Format|
|Application Note||Signal Amplification Strategies for Rapid and Sensitive High Content Analysis||PDF 776 KB|
|Data Sheet||NEL573 - 3-Amino-3-deoxydigoxigenin-9-ddGTP||PDF 54 KB|