AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Protein A conjugated AlphaScreen Acceptor beads.
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For research use only. Not for use in diagnostic procedures.
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This bead kit can be used for many applications, including:
In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The AlphaScreen Protein A Acceptor beads are used to capture an antibody. The assay is set up to bring the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction. This kit contains enough reagents to run 100 wells in 96-well format, using a 50 µL reaction volume.
|Antibody Conjugates||Streptavidin/Protein A|
|Product Brand Name||AlphaScreen|
|Quantity in a Package Amount||100.0 Units|
|Shipping Condition||Blue Ice|
|Unit Size||100 assay points|