check quantity

AlphaScreen Histidine (Nickel Chelate) Detection Kit, 500 assay points

AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and nickel chelate (Ni-NTA) AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.

Please enter valid quantity

Please login to add favorites

NULL OR EMPTY CART

Product Number Unit Size List Price Your Price Quantity
6760619C 500 assay points 170.00 USD
6760619M 10,000 assay points 2712.00 USD
6760619R 50,000 assay points 13000.00 USD

You successfully added item(s) to your cart

Overview

This bead kit can be used for many applications, including:

  • Protein-protein interaction assays
  • Protein-peptide interaction assays
  • His-tagged protein detection and quantitation
  • Protein expression
  • Binding studies

In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The other biomolecule is His-tagged, and associates with the AlphaScreen nickel chelate Acceptor beads. If the two biomolecules bind to each other, the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction. Competition formats are also possible, using the biotinylated His peptide probe provided in the kit.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Specifications

Antibody Conjugates Streptavidin/Nickel chelate
Automation Compatible Yes
Product Brand Name AlphaScreen
Shipping Condition Blue Ice
Unit Size 500 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha Technology Solutions PDF 4 MB
Guide Alpha Protein-Protein Interaction Quick Start Guide PDF 380 KB
Guide User's Guide To Alpha Assays Protein:Protein Interactions PDF 3 MB