AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and nickel chelate (Ni-NTA) AlphaScreen Acceptor beads. This kit contains enough reagents to run 500 wells in 384-well format, using a 25 µL reaction volume.
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For research use only. Not for use in diagnostic procedures.
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This bead kit can be used for many applications, including:
In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The other biomolecule is His-tagged, and associates with the AlphaScreen nickel chelate Acceptor beads. If the two biomolecules bind to each other, the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction. Competition formats are also possible, using the biotinylated His peptide probe provided in the kit.
|Antibody Conjugates||Streptavidin/Nickel chelate|
|Product Brand Name||AlphaScreen|
|Quantity in a Package Amount||10000.0 Units|
|Shipping Condition||Blue Ice|
|Unit Size||10,000 assay points|
Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA, protein:carbohydrate, protein:small molecule, receptor:ligand, and nuclear receptor:ligand interactions. Both cell-based and biochemical interactions have been monitored, and applications such as phage display, ELISA, and EMSA (electrophoretic mobility shift assay) have been adapted to Alpha.
AlphaScreen® and AlphaLISA® are bead-based assay technologies used to study biomolecular interactions in a microplate format. The acronym “Alpha” stands for Amplified Luminescent Proximity Homogeneous Assay. The assay does not require any washing steps. Binding of proteins or other binding partners captured on the beads leads to an energy transfer from one bead to the other, ultimately producing a luminescent signal.