PerkinElmer
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AlphaScreen cAMP Detection Kit, 50,000 assay points

AlphaScreen no-wash assay kit for quantitative detection of cAMP.

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Product Number Unit Size List Price Your Price Quantity
6760635D 1,000 assay points 391.00 USD
6760635M 10,000 assay points 2793.00 USD
6760635R 50,000 assay points 13400.00 USD

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Overview

AlphaScreen no-wash assay kit for quantitative detection of cAMP. In this AlphaScreen competition assay, a biotinylated cAMP tracer binds to Streptavidin-coated Donor beads, and interacts with anti-cAMP antibody conjugated to AlphaScreen Acceptor beads. When no cAMP is present, the beads come into close proximity. The excitation of the Donor beads triggers the release of singlet oxygen molecules, which diffuse to the Acceptor beads, resulting in light emission at 520-620 nm. With increasing concentrations of endogenous cAMP, fewer Donor and Acceptor beads are brought together, resulting in a decrease in signal.

 

This kit contains enough reagents to run 1,000 wells using a 25 µL reaction volume. Protocol can be found on the Certificate of Analysis.

 

 

This product replaces 6760625R.

 

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target cAMP
Automation Compatible Yes
Product Brand Name AlphaScreen
Quantity in a Package Amount 1.0 per pack
Second Messenger Release cAMP
Shipping Condition Blue Ice
Unit Size 50,000 assay points

Resources, Events & More

Resource Type File Name File Format
Event Society of Laboratory Automation & Screening (SLAS) Event