AlphaScreen® no-wash assay kit containing Streptavidin Donor beads and Anti-FLAG conjugated AlphaScreen Acceptor beads.
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For research use only. Not for use in diagnostic procedures.
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This kit contains enough reagents to run 10,000 wells in 384-well format, using a 25 µL reaction volume. The M2 antibody clone can recognize N-terminal, C-terminal, and internal FLAG tags.
This bead kit can be used for many applications, including:
In a typical AlphaScreen assay, one biomolecule of interested is biotinylated and associates with the Streptavidin-coated Donor beads. The other biomolecule is FLAG-tagged, and associates with the AlphaScreen anti-FLAG Acceptor beads. If the two biomolecules bind to each other, the Donor and Acceptor beads are brought into proximity. Excitation of the Donor beads causes the release of singlet oxygen, which diffuses and triggers the emission of light from the Acceptor beads when in proximity. The amount of light is directly proportional to the degree of interaction. Competition formats are also possible, using the biotinylated FLAG probe provided in the kit.
|Product Brand Name||AlphaScreen|
|Quantity in a Package Amount||10000.0 Units|
|Shipping Condition||Blue Ice|
|Unit Size||10,000 assay points|
Alpha has been used to study a wide variety of interactions, including protein:protein, protein:peptide, protein:DNA, protein:RNA, protein:carbohydrate, protein:small molecule, receptor:ligand, and nuclear receptor:ligand interactions. Both cell-based and biochemical interactions have been monitored, and applications such as phage display, ELISA, and EMSA (electrophoretic mobility shift assay) have been adapted to Alpha.