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AlphaPlex-545 (Tb) Human IL2 Detection Kit, 100 assay points

The AlphaPlex™ 545 (Terbium) Human IL2 Detection Kit is designed for the quantitative determination of human IL-2 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

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Part Number Unit Size List Price Your Price Quantity
AP221TB-HV 100 assay points 589.00 USD
AP221TB-C 500 assay points 1476.00 USD
AP221TB-F 5,000 assay points 9700.00 USD
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Overview

The AlphaPlex™ 545 (Terbium) Human IL2 Detection Kit is designed for the quantitative determination of human IL-2 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

Formats:

  • Our HV (100 assay point) kits allow you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

 

AlphaPlex technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaPlex assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaPlex Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 545 nm.

 

AlphaPlex reagents are intended for use in conjunction with AlphaLISA reagents in multiplexing assays.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target IL2
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaPlex
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Inflammation
Unit Size 100 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Technical Note Homogeneous Duplexing Assay for Insulin and Glucagon Quantitation Using Alpha Technology PDF  1 MB
Event Society of Laboratory Automation & Screening (SLAS) Event