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AlphaPlex-545 Human IL17 Detection Kit, 5,000 assay points

The AlphaPlex™ 545 (Terbium) Human IL17 Detection Kit is designed for the quantitative determination of human IL17 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

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Part Number Unit Size List Price Your Price Quantity
AP219TB-HV 100 assay points 348.00 USD
AP219TB-C 500 assay points 1476.00 USD
AP219TB-F 5,000 assay points 9700.00 USD
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Overview

The AlphaPlex™ 545 (Terbium) Human IL17 Detection Kit is designed for the quantitative determination of human IL17 together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

This kit contains reagents to run 100 assays, using a 100 µL reaction volume (10 µL of sample). Protocol can be found on the Certificate of Analysis.

AlphaPlex technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaPlex assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaPlex Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 545 nm.

AlphaPlex reagents are intended for use in conjunction with AlphaLISA reagents in multiplexing assays.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target IL17A
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaPlex
Quantity in a Package Amount 5000.0 Units
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Inflammation
Unit Size 5,000 assay points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Technical Note Homogeneous Duplexing Assay for Insulin and Glucagon Quantitation Using Alpha Technology PDF  1 MB
Event Society of Laboratory Automation & Screening (SLAS) Event