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AlphaPlex-645 (Sm) Human IL1β Detection Kit, 5,000 Assay Points

The AlphaPlex™ 645 (Samarium) Human IL1β Detection Kit is designed for the quantitative determination of human insulin together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

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Product Number Unit Size List Price Your Price Quantity
AP220SM-HV 100 assay points 409.00 USD
AP220SM-C 500 assay points 1433.00 USD
AP220SM-F 5,000 assay points 9500.00 USD

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Overview

The AlphaPlex™ 645 (Samarium) Human IL1ß Detection Kit is designed for the quantitative determination of human insulin together with another analyte (AlphaLISA) in serum, buffered solution or cell culture medium using a homogeneous (no wash steps, no separation steps) assay.

This kit contains reagents to run 100 assays, using a 100 µL reaction volume (10 µL of sample). Protocol can be found on the Certificate of Analysis.

AlphaPlex technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaPlex assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaPlex Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 645 nm.

AlphaPlex reagents are intended for use in conjunction with AlphaLISA reagents in multiplexing assays.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target IL1β
Assay Target Class Cytokine
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaPlex
Shipping Condition Blue Ice
Therapeutic Area Inflammation
Unit Size 5,000 assay points