PerkinElmer
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Vimentin (human) AlphaLISA Detection Kit, 500 assay points

The AlphaLISA® immunoassay kit for human vimentin enables the quantitative determination of human vimentin in serum, buffered solution, and cell culture media using a homogeneous AlphaLISA assay (no wash steps).

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Part Number Unit Size List Price Your Price Quantity
AL372HV 100 Assay Points 728.00 USD
AL372C 500 Assay Points 1747.00 USD
AL372F 5,000 Assay Points 14000.00 USD
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Overview

Formats:

  • Our 100 assay point kit allows you to run 100 wells in 96-well format, using a 100 µL reaction volume (10 µL of sample).
  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Vimentin is one of the components of cell intermediate filaments. It is a member of the class III filament proteins, and is the main cytoskeletal component of mesenchymal cells. It is also present in other non-epithelial cells It is widely used as a marker for the presence and growth of sarcoma cancers. Modified forms of vimentin (such as citrunilated forms) are also markers of arthritis and other inflammations. This kit allows for the detection of vimentin in cell culture media and cell extracts.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target Vimentin
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Target Species Human
Therapeutic Area Cancer
Unit Size 500 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Manual Manual: AlphaLISA Vimentin Detection Kit AL372 PDF  575 KB