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VEGF-C (human) AlphaLISA Detection Kit, 500 Assay Points

AlphaLISA® no-wash immunoassay kit for detection of human VEGF-C in serum, buffered solution or cell culture medium. This kit contains enough reagents to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

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Product Number List Price Your Price Quantity
AL252C 1433.00 USD

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Details

Formats:

  • Our 500 assay point kit allows you to run 500 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).
  • Our 5,000 assay point kit allows you to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample).

Features:

  • No-wash steps, no separation steps
  • ELISA alternative technology
  • Sensitive detection
  • Broad sample compatibility
  • Small sample volume
  • Results in less than 3 hours
  • Half the time of an ELISA assay

Vascular Endothelial Growth Factor C (VEGFC) is part of the VEGF family of dimeric proteins that are important regulators of physiological and pathological vasculogenesis, angiogenesis, and lymphangiogenesis. Fully processed VEGFC contains 116 amino acid residues and is a glycoprotein. VEGFC is expressed in many tissues and exerts its effect through two endothelial receptor tyrosine kinases (VEGFR2 and VEGFR3), which play a pivotal role in regulation of endothelial cell growth and differentiation. VEGFC is one of the most potent lymphangiogenic members of the VEGF family and causes enhanced metastasis to regional lymph nodes and to the lungs in breast cancer. VEGFC seems to be a useful serum biomarker in the prognosis of certain cancers.

AlphaLISA technology allows the detection of molecules of interest in a no-wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer: For research use only. Not for use in diagnostic procedures.

Assay Target VEGF-C
Assay Target Class Protein
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
One Unit Contains 500 assay points
Product Brand Name AlphaLISA
Shipping Condition Blue Ice
Therapeutic Area Angiogenesis
Unit Size 500 assay points