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AlphaLISA TruHits Biotin-free Detection Kit, 10,000 Assay Points

Bead control set for identifying false positives and interfering compounds.

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Part Number Unit Size List Price Your Price Quantity
AL901D 1,000 Assay Points 1475.00 USD
AL901M 10,000 Assay Points 9700.00 USD
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Overview

The AlphaLISA TruHits kit is designed as a tool for AlphaLISA users to identify false positives in AlphaLISA HTS assays early in the screening process, when working with biotin-rich media. This kit includes AlphaLISA Digoxigenin TruHits Acceptor and Anti-Digoxigenin Fab Fragment Donor beads which interact together to generate an AlphaLISA signal. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm. The AlphaLISA Biotin-Free TruHits kit allows the identification of light scattering molecules (insoluble compounds), singlet oxygen quenchers and Digoxigenin mimetics interfering with the AlphaLISA signal, and thus facilitates the detection of false positives. However, TruHits kits do not allow systematic identification of all interfering compounds.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Shipping Condition Blue Ice
Target Species Human
Unit Size 10,000 Assay Points

Resources, Events & More

Resource Type File Name File Format
Brochure Alpha product listing PDF  124 KB
Application Note Avoiding Biotin Interference in AlphaLISA Assays PDF  1 MB
Manual AlphaLISA Biotin-Free TruHits Kits PDF  204 KB