PerkinElmer
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AlphaLISA Two-in-One Detection Kit: Total or Oligomerized Aβ42, 5,000 assay points

The AlphaLISA® Two-in-One Amyloid Beta 42 (Aβ 1-42) Detection Kit is designed for detection and quantitation of total and oligomerized human Aβ 1-42 in cell culture supernatants and cerebrospinal fluid (CSF) using a homogeneous (no-wash steps, no separation steps) assay. The kit includes standards for both oligomerized and monomeric Aβ1-42, allowing the assay to be run for accurate quantitation. The oligomerized form is provided in collaboration with Crossbeta, using their innovative oligomer stabilization technology. 

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Product Number Unit Size
AL330C 500 assay points
AL330F 5,000 assay points

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Overview

Achieve high sensitivity with our new 2-in-1 detection kit, using Crossbeta's stabilized Aβ oligomers standard analyte.

The AlphaLISA® Two-in-One Amyloid Beta 42 (Aβ 1-42) Detection Kit is designed for detection and quantitation of total and oligomerized human Aβ 1-42 in cell culture supernatants and cerebrospinal fluid (CSF) using a homogeneous (no-wash steps, no separation steps) assay. The kit includes standards for both oligomerized and monomeric Aβ1-42, allowing the assay to be run for accurate quantitation. The oligomerized form is provided in collaboration with Crossbeta, using their innovative oligomer stabilization technology.

This kit contains enough reagents to run 5,000 wells in 96-well or 384-well format, using a 50 µL reaction volume (5 µL of sample). Amyloid beta Oligomer analyte included in this kit is provided in solution. Upon receipt, store immediately at -20°C and do not thaw analyte for any reason until use. Upon thawing, store analyte on ice, do not vortex or aggressively mix, and use within 1 hour. Each analyte sample can only be used once (do not re-freeze once thawed).

AlphaLISA technology allows the detection of molecules of interest in a no- wash, highly sensitive, quantitative assay. In an AlphaLISA assay, a biotinylated anti-analyte antibody binds to the Streptavidin-coated Donor beads while another anti-analyte antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads causes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.

Disclaimer:  For research use only. Not for use in diagnostic procedures.

Specifications

Assay Target Aβ oligomers
Assay Target Class Peptide
Automation Compatible Yes
Detection Method Alpha
Experimental Type In vitro
Product Brand Name AlphaLISA
Shipping Condition Dry Ice
Therapeutic Area Central Nervous System
Unit Size 5,000 assay points

Resources, Events & More

Resource Type File Name File Format
Data Sheet Manual: AlphaLISA Two-in-One Detection Kit: Total or Oligomerized Aß42 PDF 563 KB
Event Society of Laboratory Automation & Screening (SLAS) Event