The AlphaLISA® immunoassay kit for human TIM-3 enables the quantitative determination of human T-cell immunoglobulin and mucin-domain containing-3 (TIM-3) in buffered solution and cell culture supernatants using a homogeneous AlphaLISA assay (no wash steps).
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|Part Number||Unit Size||List Price||Your Price||Quantity|
|AL3052HV||100 Assay Points||600.00 USD|
|AL3052C||500 Assay Points||1800.00 USD|
|AL3052F||5,000 Assay Points||14400.00 USD|
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AlphaLISA technology allows the detection of molecules of interest in buffer, cell culture media, serum and plasma in a highly sensitive, quantitative, reproducible and user-friendly mode. In an AlphaLISA assay, a Biotinylated Anti-Analyte Antibody binds to the Streptavidin-coated Alpha Donor beads, while another Anti-Analyte Antibody is conjugated to AlphaLISA Acceptor beads. In the presence of the analyte, the beads come into close proximity. The excitation of the Donor beads provokes the release of singlet oxygen molecules that triggers a cascade of energy transfer in the Acceptor beads, resulting in a sharp peak of light emission at 615 nm.
TIM-3 (T-cell immunoglobulin and mucin-domain containing-3), also known as HAVCR2 (Hepatitis A virus cellular receptor 2), is an immunosuppressive protein that enhances tolerance and inhibits anti-tumor immunity. TIM-3, as an immune checkpoint marker, belongs the famility of inhibitory receptors such as PD-1 and LAG3. TIM-3 is up‑regulated on several populations of activated myeloid cells and T cells (Th1, CD8+, NK, Treg) and plays critical roles in immunosuppressive functions and in anti-tumor immunity. Its binding to Galectin-9 induces a range of immunosuppressive activities which enhance immune tolerance and inhibit anti-tumor immunity. For example, TIM-3 ligation attenuates CD8+ and Th1 cell responses and promotes the activity of Treg and myeloid derived suppressor cells. This kit is designated to detect and quantify the levels of TIM-3 in cell culture supernatant, serum, and cell lysates.
Disclaimer: For research use only. Not for use in diagnostic procedures.
|Assay Target Class||Protein|
|Product Brand Name||AlphaLISA|
|Shipping Condition||Blue Ice|
|Unit Size||100 Assay Points|
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|Resource Type||File Name||File Format|
|Brochure||Alpha product listing||PDF 128 KB|
|Application Note||Development of an AlphaLISA Assay for Screening Inhibitors of the TIM-3/Gal-9 Interaction||PDF 3 MB|
|Application Note||Protocol Optimization for Detection and Quantification of Membrane-Bound Proteins Using AlphaLISA Te||PDF 2 MB|
|Application Note||Quantifying Changes in CD28 and CTLA-4 Levels in Peripheral Blood Mononuclear Cells with AlphaLISA||PDF 1 MB|
|Application Note||Utilizing AlphaLISA Technology to Screen for Inhibitors of the CTLA-4 Immune Checkpoint||PDF 1 MB|
|Manual||AlphaLISA human TIM-3 Detection Kit||PDF 597 KB|
|Technical Note||AlphaLISA Assays for Immune Checkpoint Detection and Quantitation||PDF 532 KB|